6SP3
mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.800, 176.140, 54.370 |
Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
Resolution | 50.880 - 3.000 |
R-factor | 0.2407 |
Rwork | 0.239 |
R-free | 0.26740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sff |
RMSD bond length | 0.003 |
RMSD bond angle | 0.654 |
Data reduction software | XDS (20170615) |
Data scaling software | XDS (20170615) |
Phasing software | PHASER (2.8.0) |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.714 | 58.714 | 3.180 |
High resolution limit [Å] | 2.990 | 8.860 | 2.990 |
Rmeas | 0.078 | 0.029 | 1.358 |
Number of reflections | 19059 | 744 | 2957 |
<I/σ(I)> | 12.59 | 43.56 | 1.11 |
Completeness [%] | 98.9 | ||
Redundancy | 4.13 | 3.88 | 4.16 |
CC(1/2) | 0.999 | 0.999 | 0.646 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | 5% MPD, 10% PEG6000, 100mM HEPES pH7.5 (Crystal screen HT condition 78) |