6SNE
crystal structure of LN01 Fab in complex with an HIV-1 gp41 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.686, 144.644, 409.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.630 - 3.900 |
R-factor | 0.2201 |
Rwork | 0.218 |
R-free | 0.26060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.167 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.630 | 4.090 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.144 | 0.555 |
Number of reflections | 35232 | 3519 |
<I/σ(I)> | 7 | 2.7 |
Completeness [%] | 96.2 | |
Redundancy | 4.2 | |
CC(1/2) | 0.995 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M HEPES pH 7.5 9 % PEG 8000 8 % Ethylene glycol 10 mM Mn Cl2 |