6SMC

mouse Interleukin-12 subunit beta - p80 homodimer in space group P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1
Unit cell lengths	50.074, 96.791, 107.780
Unit cell angles	68.59, 76.84, 85.83

Refinement procedure

Resolution	48.760 - 3.500
R-factor	0.2818
Rwork	0.279
R-free	0.30980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sff
RMSD bond length	0.003
RMSD bond angle	0.682
Data reduction software	XDS (20190315)
Data scaling software	XDS (20190315)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.760	48.760	3.710
High resolution limit [Å]	3.500	10.280	3.500
Rmeas	0.233	0.058	1.852
Number of reflections	21609	821	3445
<I/σ(I)>	5.49	23.46	0.77
Completeness [%]	92.9	89.6	91.9
Redundancy	4.12	4.05	4.09
CC(1/2)	0.992	0.996	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	287	200 MM NAFORMATE, 100 MM NAPO4 PH 6.2, 10 % GLYCEROL, 20% MEDIUM PEG SMEAR (BCS CONDITION 40)