6SM5
MbC/SHP1-C-SH2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-10 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.898, 63.820, 73.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.100 - 2.750 |
R-factor | 0.224949000386 |
Rwork | 0.219 |
R-free | 0.27645 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jeg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmeas | 0.140 | 0.650 |
Number of reflections | 10640 | 1726 |
<I/σ(I)> | 7.09 | 1.73 |
Completeness [%] | 92.0 | |
Redundancy | 2.11 | |
CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium citrate tribasic, 0.1 M bistros propane 8.4 and 20 % PEG 3350 |