6SLI

Structure of the RagAB peptide transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.928
Spacegroup name	C 2 2 21
Unit cell lengths	190.560, 376.870, 369.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.920 - 3.380
R-factor	0.2068
Rwork	0.204
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cx8 5fq8
RMSD bond length	0.012
RMSD bond angle	1.455
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.920	3.440
High resolution limit [Å]	3.380	3.380
Rmeas	0.234	1.276
Rpim	0.084	0.449
Number of reflections	184493	9162
<I/σ(I)>	8.5
Completeness [%]	99.9	100
Redundancy	7.6	8.1
CC(1/2)	0.992	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	18% PEG200 0.1 M KCl 0.1 M K-phosphate