6SLH
Conformational flexibility within the small domain of human serine racemase.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.200, 155.740, 85.580 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 40.870 - 1.890 |
| Rwork | 0.172 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hmk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.859 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.870 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmeas | 0.071 | 1.086 |
| Number of reflections | 98693 | 4996 |
| <I/σ(I)> | 10.5 | 1.5 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bis-Tris pH 6.5, 15% PEG 3350, 250 mM MgCl2 |
