6SL6
p53 charged core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.423, 84.963, 84.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.481 - 1.670 |
| R-factor | 0.1642 |
| Rwork | 0.163 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xwr |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.850 | 1.730 |
| High resolution limit [Å] | 1.668 | 1.670 |
| Rmeas | 0.088 | 0.780 |
| Number of reflections | 28523 | 2713 |
| <I/σ(I)> | 15.6 | 2.58 |
| Completeness [%] | 98.2 | 95.13 |
| Redundancy | 7.8 | 7.2 |
| CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2 M Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350 |
