6SKU

Legionella effector AnkX in complex with human Rab1b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	171.350, 68.470, 137.370
Unit cell angles	90.00, 98.93, 90.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.2493
Rwork	0.247
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bes 3nkv
RMSD bond length	0.002
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.045	0.570
Number of reflections	25684	2264
<I/σ(I)>	13.9	2.3
Completeness [%]	97.5
Redundancy	3.1
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	3.5 M Sodium formate