6SKJ
DeltaC2 C-terminal truncation of HsNMT1 in complex with MyrCoA and GNCFSKPR substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.965, 58.270, 154.132 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 49.371 - 2.800 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o9v |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.738 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.371 | 49.370 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.640 | 2.600 |
| Rmerge | 0.136 | 0.036 | 1.918 |
| Rmeas | 0.149 | 0.040 | 2.117 |
| Rpim | 0.059 | 0.016 | 0.870 |
| Total number of observations | 2010 | 6956 | |
| Number of reflections | 22838 | 311 | 1371 |
| <I/σ(I)> | 9.9 | 37.4 | 0.9 |
| Completeness [%] | 89.3 | 99.3 | 74.5 |
| Redundancy | 5.9 | 6.5 | 5.1 |
| CC(1/2) | 0.996 | 0.999 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 19% PEG6K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl |
