6SK8
DeltaC3 C-terminal truncation of HsNMT1 in complex with MyrCoA and GDCFSKPR substrates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978565 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.622, 58.256, 154.789 |
Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
Resolution | 49.310 - 1.870 |
R-factor | 0.2096 |
Rwork | 0.208 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o9v |
RMSD bond length | 0.003 |
RMSD bond angle | 0.691 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.310 | 49.310 | 1.910 |
High resolution limit [Å] | 1.870 | 8.970 | 1.870 |
Rmerge | 0.159 | 0.061 | 1.219 |
Rmeas | 0.171 | 0.067 | 1.307 |
Rpim | 0.063 | 0.026 | 0.467 |
Total number of observations | 507596 | 4649 | 33696 |
Number of reflections | 68387 | 667 | 4360 |
<I/σ(I)> | 7.3 | 19.8 | 1.6 |
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 7.4 | 7 | 7.7 |
CC(1/2) | 0.994 | 0.995 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 19% PEG8K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl |