6SJD
ZC3H12B-ribonuclease domain bound to RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 114.237, 114.237, 165.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.954 - 3.290 |
R-factor | 0.2371 |
Rwork | 0.235 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v33 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 93.972 | 93.972 | 3.532 |
High resolution limit [Å] | 3.290 | 10.198 | 3.291 |
Rmerge | 0.436 | 0.072 | 5.357 |
Rmeas | 0.445 | 0.075 | 5.461 |
Rpim | 0.088 | 0.017 | 1.051 |
Number of reflections | 13344 | 666 | 668 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 94.3 | 99.6 | 58.1 |
Redundancy | 25.5 | 19.9 | 26.7 |
CC(1/2) | 0.997 | 0.997 | 0.270 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | magnesium chloride, sodium cacodilate, 2-methyl-2,4-pentandiol |