6SJ6
Cryo-EM structure of 50S-RsfS complex from Staphylococcus aureus
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.23 |
Number of Particle | 172729 |
Software | RELION,PHENIX,EPU,Coot,Gctf,MDFF |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |