6SH7

Crystal structure of the human DEAH-helicase DHX15 in complex with the NKRF G-patch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000041
Spacegroup name	C 2 2 21
Unit cell lengths	82.243, 90.024, 213.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.230 - 2.210
R-factor	0.2111
Rwork	0.209
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xdr
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.230	2.290
High resolution limit [Å]	2.210	2.210
Rmeas	0.187
Rpim	0.052	0.763
Number of reflections	39975	3912
<I/σ(I)>	8.2
Completeness [%]	99.8	98.7
Redundancy	13.1	13.5
CC(1/2)	0.998	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.1 M carboxylic acids, 0.1 M BICINE/Tris pH 8.5, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD