6SFF
mouse Interleukin-12 subunit beta - p80 homodimer in space group I41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | I 41 |
Unit cell lengths | 85.876, 85.876, 107.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.140 - 2.400 |
R-factor | 0.2329 |
Rwork | 0.229 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mj3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.567 |
Data reduction software | XDS (20190315) |
Data scaling software | XDS (20190315) |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 67.410 | 67.410 | 2.550 |
High resolution limit [Å] | 2.400 | 7.170 | 2.400 |
Rmeas | 0.125 | 0.037 | 1.551 |
Number of reflections | 15233 | 592 | 2419 |
<I/σ(I)> | 12.23 | 47.82 | 0.97 |
Completeness [%] | 99.7 | 100 | 98.5 |
Redundancy | 8.11 | 8.15 | 7.16 |
CC(1/2) | 0.998 | 1.000 | 0.555 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 287 | 120 mM monosaccharides, 50 mM Imidazole, 50 mM MES, pH6.5, 20% Ethylene glycol, 10 % PEG 8000 (MORPHEUS 1, F2) |