6SF3
Bone morphogenetic protein 10 (BMP10) in complex with extracellular domain of activin receptor-like kinase 1 (ALK1) at 2.3 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 58.269, 58.269, 311.435 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.906 - 2.300 |
R-factor | 0.215492606269 |
Rwork | 0.214 |
R-free | 0.24325 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sf1 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.850 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 311.440 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.075 | |
Number of reflections | 15103 | 1408 |
<I/σ(I)> | 7.4 | 1.6 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 14.7 | 15.1 |
CC(1/2) | 0.994 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.07 M Amino acids (0.014 M DL-Glutamic acids monohydrate; 0.014 M DL-Alanine; 0.014 M Glycine; 0.014 M DL-Lysine monohydrochloride; 0.014 M DL-Serine), 0.07 M Buffer System 3 (0.07 M Tris(base)/BICINE), 35% Precipitant Mix 4 (9% v/v MPD, 9% w/v PEG 1000, 9% w/ PEG3350) |