6SER
Crystal structure of human STARD10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97624 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.336, 101.199, 125.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.043 - 2.299 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.043 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.085 | 0.440 |
| Number of reflections | 19758 | 925 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50% (v/v) PEG 200, 100 mM Sodium phosphate dibasic/ Potassium phosphate monobasic (pH 6.2), 200 mM NaCl |
