6SC6

dAb3/HOIP-RBR apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	65.948, 87.490, 241.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.482 - 2.250
R-factor	0.2019
Rwork	0.199
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljq 6SCA
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.482	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.029	0.261
Rmeas	0.040	0.040
Number of reflections	33752	3317
<I/σ(I)>	21.22
Completeness [%]	99.0
Redundancy	2
CC(1/2)	0.999	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, sodium chloride, HEPES