6SB5
CryoEM structure of murine perforin-2 ectodomain in a pore form
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5 |
| Software | RELION,EPU,Gctf,PHENIX |
| Symmetry | C16 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
