6SAW
Chromophore binding domain of bacteriophytochrome linked diguanylyl cyclase from Idiomarina species A28L (dimeric Pfr-like state).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.004700 |
Spacegroup name | H 3 |
Unit cell lengths | 344.650, 344.650, 92.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.023 - 3.000 |
R-factor | 0.1982 |
Rwork | 0.197 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6et7 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.023 | 55.023 | 3.100 |
High resolution limit [Å] | 3.000 | 20.000 | 3.000 |
Rmerge | 0.286 | 0.081 | 1.392 |
Rmeas | 0.338 | 0.099 | 1.656 |
Total number of observations | 284153 | ||
Number of reflections | 81556 | 259 | 7629 |
<I/σ(I)> | 4.94 | 14.21 | 1.12 |
Completeness [%] | 99.5 | 92.8 | 99.4 |
Redundancy | 3.484 | 3.135 | 3.402 |
CC(1/2) | 0.956 | 0.981 | 0.268 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH6.5, 20 % PEG 5000 MME |