6S8Q

Human Brr2 Helicase Region in complex with C-tail deleted Jab1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91842
Spacegroup name	P 21 21 21
Unit cell lengths	99.480, 118.632, 187.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.080 - 2.391
R-factor	0.1812
Rwork	0.179
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.390	2.400
Rmeas	0.144	1.446
Number of reflections	88725	14026
<I/σ(I)>	11.3
Completeness [%]	99.8
Redundancy	8.9
CC(1/2)	0.997	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350