6S1K
E. coli Core Signaling Unit, carrying QQQQ receptor mutation
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 8.38 |
| Software | MDFF |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
