6S16

T. thermophilus RuvC in complex with Holliday junction substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	293
Detector technology	CCD
Collection date	2015-09-02
Detector	RAYONIX MX225-HS
Wavelength(s)	0.895000
Spacegroup name	P 32 2 1
Unit cell lengths	108.066, 108.066, 133.281
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.296 - 3.409
R-factor	0.1836
Rwork	0.181
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ld0
RMSD bond length	0.010
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	3.500
High resolution limit [Å]	3.400	3.400
Rmeas	0.083	0.110
Number of reflections	12660	2003
<I/σ(I)>	16.8
Completeness [%]	99.5
Redundancy	5.6
CC(1/2)	1.000	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.4 M ammonium phosphate