6S16
T. thermophilus RuvC in complex with Holliday junction substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2015-09-02 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 0.895000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 108.066, 108.066, 133.281 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.296 - 3.409 |
R-factor | 0.1836 |
Rwork | 0.181 |
R-free | 0.23090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ld0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.165 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.300 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmeas | 0.083 | 0.110 |
Number of reflections | 12660 | 2003 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.5 | |
Redundancy | 5.6 | |
CC(1/2) | 1.000 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.4 M ammonium phosphate |