6RZ3
Crystal structure of a complex between the DNA-binding domain of p53 and the carboxyl-terminal conserved region of iASPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97620 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.090, 71.090, 255.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.800 - 4.230 |
| R-factor | 0.2474 |
| Rwork | 0.243 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vge 2xwr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.400 |
| High resolution limit [Å] | 4.230 | 4.230 |
| Rmerge | 0.158 | |
| Number of reflections | 4819 | 329 |
| <I/σ(I)> | 8.3 | 1.4 |
| Completeness [%] | 93.1 | 94.7 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 18% (w/v) polyethylene glycol 3350, 0.18 M tri-sodium citrate |
