6RX3

Crystal structure of human syncytin 2 in post-fusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	91.416, 91.416, 69.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.700 - 2.200
R-factor	0.216
Rwork	0.214
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rx1
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS (v2016)
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.118	0.198
Rmeas	0.130	0.216
Rpim	0.053	0.873
Number of reflections	17310	1474
<I/σ(I)>	11.5	1.1
Completeness [%]	99.5	99
Redundancy	11.3	11.5
CC(1/2)	1.000	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Tris pH 8.5, 25% v/v tert-butanol