6RX1

Crystal structure of human syncytin 1 in post-fusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.070638
Spacegroup name	H 3 2
Unit cell lengths	50.416, 50.416, 260.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.217
Rwork	0.216
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y4m
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	XDS (v2016)
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.120	1.250
Rmeas	0.150	1.500
Rpim	0.080	0.810
Number of reflections	7752	622
<I/σ(I)>	7	1.1
Completeness [%]	99.1	99.9
Redundancy	5.8	6.1
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M HEPES pH 7.5, 30% v/v 2-propanol, 0.2 M MgCl2