6RUS

Structure of a functional properdin monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	183.970, 47.110, 122.930
Unit cell angles	90.00, 119.41, 90.00

Refinement procedure

Resolution	45.583 - 2.800
R-factor	0.2411
Rwork	0.238
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	electron density for FP TB-TSR1-TSR5-TSR6
RMSD bond length	0.007
RMSD bond angle	1.153
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	22273	1171
<I/σ(I)>	11.3
Completeness [%]	96.1	69.8
Redundancy	5.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	1.1 M ammonium sulfate, 0.1 M sodium acetate pH 6.0, 1.2%(w/v) myo-inositol