6RTI
X-ray structure of human glutamate carboxypeptidase II (GCPII) in complex with aptamer A9g
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.096, 121.096, 216.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.660 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oot |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.636 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 105.660 | 105.660 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.164 | 0.046 | 0.985 |
| Rmeas | 0.172 | 0.050 | 1.026 |
| Rpim | 0.048 | 0.016 | 0.284 |
| Number of reflections | 82266 | 4499 | 4051 |
| <I/σ(I)> | 10.1 | 31.4 | 2 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 12.6 | 11.9 | 12.9 |
| CC(1/2) | 0.838 | 0.734 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 Mg Acet, 40% (v/v) MPD, 1 mM 2-PMPA |
