6RT7
The YTH domain of YTHDC1 protein in complex with Gm6ACU oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.850, 103.460, 42.007 |
| Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
| Resolution | 40.596 - 1.730 |
| R-factor | 0.2232 |
| Rwork | 0.220 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.821 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.610 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmeas | 0.090 | 1.114 |
| Number of reflections | 33768 | 5348 |
| <I/σ(I)> | 9.19 | |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 3.38 | 3.31 |
| CC(1/2) | 0.997 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Bis-Tris at pH 6.5, 0.2 M ammonium sulfate and 25% PEG 3350 |
