6RT5
The YTH domain of YTHDC1 protein in complex with Gm6AC oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.787, 103.655, 41.779 |
| Unit cell angles | 90.00, 104.76, 90.00 |
Refinement procedure
| Resolution | 40.400 - 2.303 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.911 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.410 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.043 | 0.147 |
| Number of reflections | 14246 | 2119 |
| <I/σ(I)> | 26.26 | |
| Completeness [%] | 97.8 | 91.1 |
| Redundancy | 4.48 | 4.5 |
| CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.1 M Bis-Tris at pH 6.5, 0.2 M ammonium sulfate and 30% PEG 3350 |
