6RRT
T=4 MS2 Virus-like-particle
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | FEI FALCON I (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6 |
| Software | EMAN,RELION,EPU,CTFFIND,UCSF Chimera |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
