6RRK
Crystal structure of the central region of human cohesin subunit STAG1 in complex with RAD21 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.968 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.333, 86.290, 118.585 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 60.229 - 3.170 |
R-factor | 0.2215 |
Rwork | 0.219 |
R-free | 0.27830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pk7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.518 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.670 | 3.390 |
High resolution limit [Å] | 3.170 | 3.170 |
Rmerge | 0.066 | 0.391 |
Rpim | 0.046 | 0.265 |
Number of reflections | 24764 | 4500 |
<I/σ(I)> | 7.3 | 1.9 |
Completeness [%] | 97.0 | 97.8 |
Redundancy | 2.8 | 2.8 |
CC(1/2) | 0.990 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 16 % PEG 3350, 0.2 M DL-Mallic acid |