6RPC
GOLGI ALPHA-MANNOSIDASE II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.525, 90.939, 84.249 |
| Unit cell angles | 90.00, 101.63, 90.00 |
Refinement procedure
| Resolution | 82.660 - 1.690 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bub |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.660 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.660 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 126315 | 819 |
| <I/σ(I)> | 11.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.998 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Sodium Succinate 10 % PEG 3350 with microseeding |
