6RP5
Crystal structure of monocarboxylated hemoglobin from the sub-Antarctic fish Eleginops maclovinus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-07 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 91.702, 91.702, 168.716 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 79.420 - 1.490 |
R-factor | 0.16974 |
Rwork | 0.168 |
R-free | 0.19577 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4esa |
RMSD bond length | 0.022 |
RMSD bond angle | 2.136 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.420 | 1.530 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.095 | 0.440 |
Rpim | 0.057 | 0.295 |
Number of reflections | 66710 | 5202 |
<I/σ(I)> | 8 | |
Completeness [%] | 96.4 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 277 | CO atmosphere , 20 mg/ml protein, 1.8 M ammonium sulfate |