6ROY
Structure of the N-SH2 domain of the human tyrosine-protein phosphatase non-receptor type 11 in complex with the phosphorylated immune receptor tyrosine-based inhibitory motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00002 |
Spacegroup name | P 43 |
Unit cell lengths | 29.856, 29.856, 208.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.060 - 2.100 |
R-factor | 0.22794 |
Rwork | 0.225 |
R-free | 0.27850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tkz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.376 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.060 | 2.160 |
High resolution limit [Å] | 2.095 | 2.095 |
Rmerge | 0.053 | 1.149 |
Rpim | 0.079 | 0.484 |
Number of reflections | 10722 | 889 |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 12.8 |
CC(1/2) | 0.946 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | 31.7% (w/v) PEG3350, 0.1 M HEPES, 0.233 M MgSO4 |