6ROY
Structure of the N-SH2 domain of the human tyrosine-protein phosphatase non-receptor type 11 in complex with the phosphorylated immune receptor tyrosine-based inhibitory motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.856, 29.856, 208.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.060 - 2.100 |
| R-factor | 0.22794 |
| Rwork | 0.225 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tkz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.060 | 2.160 |
| High resolution limit [Å] | 2.095 | 2.095 |
| Rmerge | 0.053 | 1.149 |
| Rpim | 0.079 | 0.484 |
| Number of reflections | 10722 | 889 |
| <I/σ(I)> | 11.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 12.8 |
| CC(1/2) | 0.946 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | 31.7% (w/v) PEG3350, 0.1 M HEPES, 0.233 M MgSO4 |
