6RNG
Dipeptide Gly-Pro binds to a glycolytic enzyme fructose bisphosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07227 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.399, 73.164, 176.225 |
| Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
| Resolution | 46.230 - 2.150 |
| R-factor | 0.24596 |
| Rwork | 0.243 |
| R-free | 0.29646 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ky6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.477 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.230 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rpim | 0.088 | |
| Number of reflections | 69691 | 3615 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 96.8 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium Sulfate, 0.1 M Bis-Tris pH 6.4, 30% PEG 3350 |
