6RMU
Crystal structure of disulphide-linked human C3d dimer in complex with Staphylococcus aureus complement subversion protein Sbi-IV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.518, 115.169, 118.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.733 - 2.400 |
R-factor | 0.1589 |
Rwork | 0.156 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wy7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.102 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.930 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.132 | 0.587 |
Rpim | 0.060 | 0.266 |
Number of reflections | 40413 | 4153 |
<I/σ(I)> | 8.5 | 2.8 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 5.7 | 5.8 |
CC(1/2) | 0.994 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.07 | 291 | 0.2 M sodium citrate tribasic dihydrate, 20% PEG 3350. |