6RKC
Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.372, 79.467, 143.787 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.560 |
| R-factor | 0.26136 |
| Rwork | 0.260 |
| R-free | 0.28409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rjs |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.215 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.780 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.076 | 0.612 |
| Number of reflections | 100906 | 4614 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.996 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Mg Formate and 12% PEG 3350 |
