6RFX
Crystal structure of Eis2 from Mycobacterium abscessus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.450, 79.800, 152.680 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 49.596 - 1.900 |
| R-factor | 0.2027 |
| Rwork | 0.202 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.15rc3_3435: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.130 | |
| Number of reflections | 212722 | 30174 |
| <I/σ(I)> | 11.09 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | .2 M Ammonium acetate, 0.1 M Sodium citrate pH 5.5, 24% w/v PEG 4000 and 5 mM Hygromycin |
