6RCX

Mycobacterial 4'-phosphopantetheinyl transferase PptAb in complex with the ACP domain of PpsC.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	P 21 21 21
Unit cell lengths	50.142, 63.167, 108.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.150 - 2.000
R-factor	0.2084
Rwork	0.206
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u89
Data reduction software	TRUNCATE
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.150	2.072
High resolution limit [Å]	2.000	2.000
Number of reflections	23870	12069
<I/σ(I)>	14.2
Completeness [%]	99.6
Redundancy	5.4
CC(1/2)	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	285	0.1 M Na Cacodylate 30 % PEG 8K 0.2 M (NH4)2SO4