6RCN
Crystal structure of REXO2-D199A-dAdA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2824 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 35.840, 126.890, 168.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.750 - 2.250 |
R-factor | 0.213 |
Rwork | 0.212 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.750 | 21.750 | 2.380 |
High resolution limit [Å] | 2.250 | 6.670 | 2.250 |
Rmerge | 0.071 | 0.038 | 1.390 |
Rmeas | 0.077 | 0.041 | 1.504 |
Total number of observations | 241414 | ||
Number of reflections | 35196 | 1349 | 5631 |
<I/σ(I)> | 14.15 | 46.58 | 1.17 |
Completeness [%] | 99.7 | 99.4 | 98.6 |
Redundancy | 6.859 | 6.719 | 6.807 |
CC(1/2) | 0.999 | 0.999 | 0.536 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 100 mM Hepes pH 7.0, 200 mM sodium malonate, and 20 % polyethylene glycol 3350 |