6RB4

Crystal structure of the Pri1 subunit of human primase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980110
Spacegroup name	P 43 21 2
Unit cell lengths	78.659, 78.659, 148.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.498 - 1.500
R-factor	0.1941
Rwork	0.193
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bpu
RMSD bond length	0.010
RMSD bond angle	0.844
Data reduction software	XDS
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	1.554
High resolution limit [Å]	1.500	1.500
Rmerge		0.543
Rmeas	0.027
Number of reflections	75113	7355
<I/σ(I)>	15.76	1.49
Completeness [%]	99.9	99.35
Redundancy	2	2
CC(1/2)	1.000	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100mM Bis Tris propane pH 6.5, 24 % PEG 3350, 150 mM NaF