6RA9
Novel structural features and post-translational modifications in eukaryotic elongation factor 1A2 from Oryctolagus cuniculus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-11-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979260 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 133.520, 133.520, 305.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.820 - 2.700 |
R-factor | 0.1811 |
Rwork | 0.179 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c0s |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.170 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 1.069 |
Rmeas | 0.125 | 1.118 |
Rpim | 0.034 | 0.320 |
Number of reflections | 83606 | 4339 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 | 11.2 |
CC(1/2) | 0.995 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 50 mM Na citrate pH 5/MES pH 5.6, 2.2-2.6 M ammonium sulphate, 10 mM Mg acetate |