6R8T

Crystal structure of aIF2gamma subunit I181T from archaeon Sulfolobus solfataricus complexed with GDPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-09
Detector	Bruker Platinum 135
Wavelength(s)	1.54179
Spacegroup name	I 2 3
Unit cell lengths	186.585, 186.585, 186.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.387 - 2.100
R-factor	0.1828
Rwork	0.182
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rjl
RMSD bond length	0.005
RMSD bond angle	1.102
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.390	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.343	0.107
Number of reflections	62755
<I/σ(I)>	8.02
Completeness [%]	99.9
Redundancy	3.41

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		301	15,4 mM TRIS-HCL pH7,5, 30.8mM NaCl, 3.1mM 2-mercaptoethanol, 1,1 M sodium formate, 30.8mM sodium cacodylate pH 6.5, 1,15% MMEPEG 5000, 1,4mM GDPCP