6R8S
Crystal structure of aIF2gamma subunit I181K from archaeon Sulfolobus solfataricus complexed with GDPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 186.858, 186.858, 186.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.714 - 2.180 |
| R-factor | 0.1675 |
| Rwork | 0.166 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nbs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.714 |
| High resolution limit [Å] | 2.180 |
| Number of reflections | 56432 |
| <I/σ(I)> | 14.24 |
| Completeness [%] | 100.0 |
| Redundancy | 11.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 301 | 17 mM TRIS-HCL pH7,5, 17 mM NaCl, 3,4mM 2-mercaptoethanol, 1,22 M sodium formate, 33,9 mM sodium cacodylate pH 6.5, 0,33% MMEPEG 5000, 1,7mM GDPCP |
