6R7V
Tannerella forsythia promirolysin mutant E225A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.2816 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.330, 67.250, 79.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.340 - 1.400 |
R-factor | 0.161 |
Rwork | 0.161 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r7u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.400 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 50157 | 7077 |
<I/σ(I)> | 13.8 | 1.8 |
Completeness [%] | 98.7 | |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Promirolysin crystals were obtained at 20 degrees from drops containing 200 nL of protein solution at 0.6 mg/mL in 5 mM Tris-HCl pH 8.0, 50 mM sodium chloride and 100 nL of reservoir solution, which comprised 25% PEG 1500, 0.1 M MIB buffer (malonic acid, imidazole and boric acid at a 2:3:3 molar ratio), pH 6.0. |