6R7T
Crystal Structure of human Melanoma-associated antigen B1 (MAGEB1) in complex with nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 61.976, 61.976, 361.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.673 - 2.682 |
| R-factor | 0.2594 |
| Rwork | 0.258 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wa0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.520 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.673 | 2.810 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.135 | |
| Number of reflections | 12641 | 1618 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.056M sodium phosphate monobasic - 1.344M potassium phosphate dibasic |
