6R6E
Crystal structure of di-phosphorylated human CLK1 in complex with 5-(6,7-dichloro-1-methyl-1H-indol-3-yl)pyrimidin-4-amine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.072 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 67.710, 67.710, 283.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.490 - 2.250 |
R-factor | 0.20869 |
Rwork | 0.206 |
R-free | 0.25782 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qty |
RMSD bond length | 0.017 |
RMSD bond angle | 1.793 |
Data reduction software | MOSFLM |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.490 | 2.308 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 19445 | 1839 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.9 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 24% PEG 3350, 200mM MgCl2, 100mM BisTris (pH 6.5) |