6R4T

Crystal structure of the Pri1 subunit of human primase bound to vidarabine triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97867
Spacegroup name	C 2 2 21
Unit cell lengths	111.090, 118.970, 150.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.690 - 2.350
R-factor	0.2108
Rwork	0.209
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rb4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260_000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.691	2.490
High resolution limit [Å]	2.350	2.350
Number of reflections	41814	6609
<I/σ(I)>	15.35	1.63
Completeness [%]	99.7	98.7
Redundancy	7.2
CC(1/2)	0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	23% PEG3350, 10% ethylene glycol, 200 mM Na/K tartrate