6R2U
Zinc-alpha2-Glycoprotein with a Fluorescent Dansyl C 11 Fatty Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-01-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | I 4 |
Unit cell lengths | 167.015, 167.015, 204.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.400 - 2.490 |
R-factor | 0.17126 |
Rwork | 0.170 |
R-free | 0.21202 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t7v pdbredo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.473 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.400 | 2.530 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmeas | 0.212 | 3.388 |
Rpim | 0.054 | 0.856 |
Number of reflections | 98002 | 4802 |
<I/σ(I)> | 10 | 1 |
Completeness [%] | 100.0 | 99.2 |
Redundancy | 15.3 | 15.6 |
CC(1/2) | 0.995 | 0.298 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 1.3 M (NH4)2SO4, 100 mM Hepes |