6R2S
The structure of Plasmodium vivax Duffy binding protein (PvDBP) bound to human antibody DB9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 173.560, 173.560, 169.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.040 - 3.040 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4NUV and 3DIF |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.040 | 3.090 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 30707 | 1442 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 45 % v/v polypropylene glycol 400, 10% v/v ethanol |
